ABSTRACT
The present study evaluated the anti-inflammatory and analgesic properties of Agave sisalana Perrine in classic models of inflammation and pain. The hexanic fraction of A. sisalana (HFAS) was obtained by acid hydrolysis followed by hexanic reflux. Anti-inflammatory properties were examined in three acute mouse models (xylene ear oedema, hind paw oedema and pleurisy) and a chronic mouse model (granuloma cotton pellet). The antinociceptive potential was evaluated in chemical (acetic-acid) and thermal (tail-flick and hot-plate test) models of pain. When given orally, HFAS (5, 10, 25 and 50 mg/kg) reduced ear oedema (p < 0.0001; 52%, 71%, 62% and 42%, respectively). HFAS also reduced hind paw oedema at doses of 10 mg/kg and 25 mg/kg (p < 0.05; 42% and 58%, respectively) and pleurisy at doses of 10 mg/kg and 25 mg/kg (41% and 50%, respectively). In a chronic model, HFAS reduced inflammation by 46% and 58% at doses of 10 mg/kg and 25 mg/kg, respectively. Moreover, this fraction showed analgesic properties against the abdominal writhing in an acetic acid model (at doses of 5-25 mg/kg) with inhibitory rates of 24%, 54% and 48%. The HFAS also showed an increased latency time in the hot-plate (23% and 28%) and tail-flick tests (61% and 66%) for the 25 mg/kg and 50 mg/kg doses, respectively. These results suggest that HFAS has anti-inflammatory and analgesic properties.
Subject(s)
Animals , Male , Mice , Rats , Agave/chemistry , Analgesics/therapeutic use , Anti-Inflammatory Agents/therapeutic use , Inflammation/drug therapy , Pain/drug therapy , Plant Extracts/therapeutic use , Analgesics/isolation & purification , Anti-Inflammatory Agents/isolation & purification , Carrageenan , Edema/chemically induced , Edema/drug therapy , Inflammation/chemically induced , Pain Measurement , Pain/chemically inducedABSTRACT
Desde que o magnésio foi introduzido na terapia há aproximadamente 30 anos, inúmeros estudos clínicos deveriam ter sido feitos. Embora um grande número de resultados experimentais tem demonstrado a importância do magnésio em eventos biológicos, o interesse pela terapia com magnésio ainda caminha a passos lentos, podendo ser atribuído principalmente às restriçöes dirigidas à prevençäo de doenças, particularmente aquelas associadas ao sistema imune. Entretanto, as aplicaçöes promissoras da terapia com magnésio vêm progredindo à luz da dedicaçäo de grupos de pesquisa de excelência, fazendo que a significância do magnésio näo seja ignorada. O propósito deste trabalho é o de resumir alguns aspectos relevantes da importância biológica deste cátion, com especial atençäo ao seu papel nos mecanismos de resistência e imunocompetência.